Study on bovine leukemia in dairy cattle by using cytochemical staining technique
1999
Atchariya Sailasut | Wimon Phothiwong (Chulalongkorn Univ., Bangkok (Thailand))
Study on Bovine Leukemia in dairy cattle had been performed. The blood samples from eighty, above -3-year-old dairy cattle were collected from checking the prevalence of Bovine Leukemia infection in the herd. The cows which having serologically positive to Agar Gel Immunodiffusion Test (AGID) were at 22.50 percent (18/80). The hematological studies were done in the studied groups during 6 months of observation. The total leukocyte and lymphocyte count from both groups were in normal limit and revealed no statistically difference (P0.05) between the two groups. Both blood and buffy coat smears by Wright giemsa stain demonstrated neither blast cell not lymphoblast. Cytochemical studydies of the blood and fuffy coat smear from both groups were similar in appearance. Cytochemical reactions of Sudan Black B (SBB). Periodic Acid Schiff (PAS) and Acid Phosphatase (AcP) showed the positivity as in normal haemic cells in blood circulation : SBB positivity to granulocytes, PAS positivity to granulocytes and lymphocytes and AcP positivity predominantly lymphocytes and some granulocytes. These obtained results would provide basic knowledges for the clinical applications of the hemopoietic diseases in domestic animals.
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