[Action of hydrogen peroxide on the intracellular content of calcium ions in the thymocytes of rats]
2006
Pukhteeva, I.V. | Prokopenko, N.V. | Gerasimovich, N.V.(The A.D. Sakharov International State Ecological Univ., Minsk (Belarus))
The role of free radicals in functioning of the biological systems was analyzed. The ability of active forms of oxygen to damage the biological molecules and their role in the disorganization of the cellular structures was shown. Results of the correlation analysis of cytoplasm calcium ions and intra-cellular calcium depot of thymocytes of rats after the influence of various concentrations of hydrogen peroxide (10(sup-9)-10(sup-3) mole/l) were presented. The structural state of the plasmatic membrane was analyzed. Research results of cytoplasmic free calcium showed that under the influence of various concentrations of exogenous hydrogen peroxide there was the increase of Ca2+ in the cytoplasm. Analysis of the quantity of membrane-bound calcium ions in the depot of thymocytes showed the time-depended reduction of the observed ions. The influence of exogenous hydrogen peroxide on thymus cells caused the modification of structural state of the lipidic component of plasmatic membranes. Degree of manifestation of the observed influence had the direct relation with the concentration of hydrogen peroxide and time of its impact. Research results showed that the physiological concentrations hydrogen peroxide (10(sup-9)-10(sup-8) mole/l) were nontoxic for cells because they did not cause the change of homeostasis of calcium ions and physical and chemical state of plasmalemma. Investigation results testified that hydrogen peroxide had the pluripotential effect on the functional state of cells
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