Effects of L-arginine and L-cysteine on growth, and chlorophyll and mineral contents of shoots of the apple rootstock EM 26 cultured in vitro
Sotiropoulos, T.E.(Pomology Inst., Naoussa (Greece))E-mail:[email protected] | Dimassi, K.N.(Aristotle Univ. of Thessaloniki (Greece). Lab. of Pomology) | Therios, I.N.(Aristotle Univ. of Thessaloniki (Greece). Lab. of Pomology)
One, five, or ten mM arginine and 25, 50, or 100 M cysteine were added in the Murashige and Skoog medium. By increasing arginine concentration the number of shoots per explant increased. Inclusion of 50 M cysteine in the medium resulted in maximum number of shoots but it was not significantly different in comparison with 10 mM arginine. The chlorophyll content was significantly increased in explants treated with 10 mM arginine in comparison with the control, 1 mM arginine and 25 M cysteine. By increasing arginine and cysteine concentrations of the medium, N, K, and Ca concentrations of explants increased but no significant changes in P, Mg, Fe, Mn, Zn, and B concentrations were observed.
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