Effects of ammonia treatment under various conditions on viability of Mycobacterium avium subsp. paratuberculosis inoculated in low moisture roughage
2004
Katayama, N. (Shizuoka-ken. Animal Husbandry Experiment Station, Fujinomiya (Japan)) | Shibata, M. | Suzuki, T. | Ootake, M. | Kamata, S. | Yokomizo, Y.
We investigated the effects of ammonia treatment under various conditions on the viability of Mycobacterium avium subsp. paratuberculosis (MAP), which contaminates low moisture roughage. A small resin bag called a pouch silo and containing ground alfalfa hay was used as a container for ammonia treatment. The ammonia treatment was performed on a laboratory scale with NH4 OH and a filter paper infiltrating cultured MAP suspension. At a 3% concentration, except for the 1st day under 10 deg C and 20% moisture conditions, no surviving MAP was observed within 3 days after addition of ammonia. When treated with 1 and 2% concentrations of ammonia, MAP was observed to survive under conditions of low temperature and low moisture. The correlation coefficients between viability and material moisture, processing temperature, ammonia concentration, and the number of treatment days were determined to be weak at -0.39, -0.38, -0.34, and -0.26, respectively. The result suggests that hay contaminated with MAP could be disinfected by ammonia treatment under fixed conditions.
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