Effects of temperature and light on stability of microalgal viruses, HaV, HcV and HcRNAV
2005
Tomaru, Y. (National Research Inst. of Fisheries and Environment of Inland Sea, Ohno, Hiroshima (Japan)) | Tanabe, H. | Yamanaka, S. | Nagasaki, K.
Viruses infectious to the fish-killing raphidophyte Heterosigma akashiwo (Hada) Hada (Heterosigma akashiwo Virus (HaV)) and the bivalve-killing dinophyte Heterocapsa circularisquama Horiguchi (Heterocapsa circularisquama Virus (HcV) and Heterocapsa circularisquama RNA Virus (HcRNAV)) have recently been isolated and characterized. The discovery of these microalgal viruses allows for their assessment as potential microbial agents for controlling harmful algal blooms (HABs). To assess the possibility of their practical use as anti-HAB agents, however, it is necessary to estimate how stable their infectivity is both in situ and in vitro. In the present study, we measured the effects of light and temperature on the stability of these viruses. All three viruses were susceptible to cool white fluorescent illumination. Although significant loss of infectivity at 4 deg C in the dark for one week was detected in HaV and HcV, the infectivity of HcRNAV was stable for more than one year under the same storage conditions. Furthermore, we succeeded in developing cryopreservation methods for HcV and HcRNAV, which would be useful for their long-term storage.
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