Enhanced undecylprodigiosin production from Serratia marcescens SS-1 by medium formulation and amino-acid supplementation
2005
Wei, Y.H.(Yuan-Ze Univ., Taoyuan (Taiwan)) | Yu, W.J. | Chen, W.C.
Serratia marcescens Simon Swift-1 (SS-1) was used to produce a prodigiosin-like pigment, undecylprodigiosin (UP), known to have antitumor activities and potential as an anticancer drug. Modified media containing components of Luria-Bertani (LB) broth and selected amino acids were used to improve UP production from S. marcescens SS-1. Optimal culture conditions (e.g., temperature, pH, agitation rate) for UP production were also identified. It was found that S. marcescens SS-1 was able to produce 690 mg l(-1) of UP when it was grown with 5 g l(-1) yeast extract alone (YE medium) under the optimal culture conditions of 30 deg C, 200 rpm, and pH 8. The UP production of 690 mg l(-1) is nearly 23-fold of that obtained from original LB medium. Addition of amino acids containing pyrrole-like structures further enhanced UP production. Nearly 2 and 1.4 g l(-1) of UP was produced when the SS-1 strain was cultivated with YE medium supplemented with proline and histidine (5 g l(-1)), respectively. Moreover, the addition of aspartic acid (5 g l(-1)) also resulted in a high UP production of 1.4 g l(-1). Optimal dosages of the three amino acids were subsequently determined and the highest UP production (2.5 g l(-1)) was achieved with the addition of 10 g l(-1) of proline. This suggests that the supplementation of amino acids related to the formation of a UP precursor (e.g., pyrrolylpyrromethene) could enhance UP production by the SS-1 strain.
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