Amylase production in solid state fermentation by the thermophilic fungus Thermomyces lanuginosus
2005
Kunamneni, A.(Durban Inst. of Technology (South Africa)) | Permaul, K. | Singh, S.
The production of extracellular amylase by the thermophilic fungus Thermomyces lanuginosus was studied in solid state fermentation (SSF). Solid substrates such as wheat bran, molasses bran, rice bran, maize meal, millet cereal, wheat flakes, barley bran, crushed maize, corncobs and crushed wheat were studied for enzyme production. Growth on wheat bran gave the highest amylase activity. The maximum enzyme activity obtained was 534U/g of wheat bran under optimum conditions of an incubation period of 120h, an incubation temperature of 50deg C, an initial moisture content of 90%, a pH of 6.0, an inoculum level of 10% (v/w), a salt solution concentration of 1.5:10(v/w) and a ratio of substrate weight to flask volume of 1: 100 with soluble starch (1%w/w) and peptone (1%w/w) as supplements.
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