Molecular characterization of Banana bunchy top virus and Cucumber mosaic virus from abaca (Musa textilis Nee)
2006
Furuya, N.(Tokyo Univ. of Agriculture (Japan)) | Dizon, T.O. | Natsuaki, K.T.
Abaca (Musa textilis Nee), a fiber crop, is considered one of principal industrial-crops in the Philippines. This study was conducted to clarify the occurrence and the molecular characterization of Banana bunchy top virus (BBTV), Cucumber mosaic virus (CMV), and Banana bract mosaic virus (BBrMV) in abaca and banana plants collected in Luzon and Negros islands, the Philippines. In abaca, we detected BBrMV from 34, BBTV from 12, and CMV from 6 out of 37 samples. In banana, we confirmed BBTV from 19, BBrMV from 14, and CMV from 3 out of 25 samples. These results revealed that BBrMV for abaca, BBTV and BBrMV for banana were major viruses in the studied area, although abaca bunchy top was recognized as the most serious disease economically. Several abaca varieties were evaluated for their resistance against bunchy top disease and found some hybrids to show resistant reaction. The putative causal virus of abaca bunchy top disease was partially sequenced, and the 3 genes of the replication initiation protein, the coat protein, and the movement protein were compared with those of related viruses previously reported. As the virus isolated from abaca plants showed more than 99% of homologies with BBTVs isolated from banana plants by comparison of the putative amino acid sequences of the each genes, both isolates were shown as identical in molecular characterization. Moreover, this study showed the first detection of CMV from abaca by ELISA and RT-PCR. Based on the sequence analysis of the coat protein gene, one abaca isolate of CMV was classified as subgroup IB.
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