Cold Shock Response of Leuconostoc mesenteroides SY1 Isolated from Kimchi
2005
Kim, J.H. (Gyeongsang National University, Jinju, Republic of Korea) | Park, J.Y. (Gyeongsang National University, Jinju, Republic of Korea) | Jeong, S.J. (Gyeongsang National University, Jinju, Republic of Korea) | Chun, J.Y. (Gyeongsang National University, Jinju, Republic of Korea) | Kim, J.H. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: [email protected]
Low-temperature adaptation and cryoprotection were studied in Leuconostoc mesenteroides SY1, a strain isolated from Kimchi. L. mesenteroides SY1 cells grown in exponential growth phase at 30℃ were exposed to 15℃, 10℃, and 5℃ for 2, 4, and 6 h, respectively, and then frozen at -70℃ for 24 h. Survival ratio was measured after the cells were thawed. The freezing-thawing cycles were repeated four times. Preadapted cells survived better than non-adapted control cells, and the highest survival ratio (96%) was observed for cells preadapted for 2 h at 5℃, whereas control cells showed only 22%.
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