Study on DNase activity specific from Haemonchus contortus reproductive tissue
Kwak, D.M.(Washington State University, Pullman, WA, USA)E-mail:dmkwak@vetmed.wsu.edu
DNase activity in Haemonchus contortus reproductive tissue was characterized and compared to that in whole worm. DNase activity in reproductive tissue was detected throughout pHs 4-10 with high activity under acidic conditions. The activity was not inhibited by 10 mM EDTA at pH 5.0, but largely inhibited by pH 7.0. The activity produced DNA fragments with mixtures of 3'-hydroxyls (OH) and 3'-phosphates (P) at each pH. Three distinct DNase activities were identified and had M∧rs of 34, 36 and 38.5 kDa in zymograms, which were distinguished according to pH requirement and sensitivity to EDTA.
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