Statistical Approach to Development of Culture Medium for Ansamitocin P-3 Production with Actinosynnema pretiosum ATCC 31565
2005
Bandi, Srinivasulu (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea) | Kim, Y.J. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea) | Sa, S.O. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea) | Chang, Y.K. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea), E-mail: [email protected]
The Plackett-Burman design and the response surface method (RSM) with a central composite design (CCD) were employed to develop a culture medium for ansamitocin P-3 production using Actinosynnema pretiosum ATCC 31565. Among the 11 nutrients tested using the Plackett-Burman design, two carbon sources, sucrose and dextrin, and two nitrogen sources, polypeptone and yeast extract, were selected. Optimization of the concentrations of the selected nutrients was then performed using RSM with CCD.
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