Development of Serum-Free Media for Primary Culture of Human Articular Chondrocytes
2005
Choi, Y.S. (Inha University, Incheon, Republic of Korea) | Lim, S.M. (Inha University, Incheon, Republic of Korea) | Lee, C.W. (Good Shepherd Hospital, Seoul, Republic of Korea) | Kim, D.I. (Inha University, Incheon, Republic of Korea), E-mail: [email protected]
Human articular chondrocytes (HAC) were cultivated as a monolayer in a serum-free medium for primary culture (SFM-P). An optimized SFM-P provides 95% proliferation rate of the obtainable from primary and secondary chondrocyte cultures grown in a control medium with serum. The gradual decrease in the amounts of synthesized glycosaminoglycan and type Ⅱ collagen was improved by coating the culture dishes with type Ⅳ collagen and fibronectin.
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