Effect of IRES Controlled Reporter Gene on Screening and Production of Recombinant Human EPO Proteins from Cultured CHO Cells
2006
Lee, H.G. (Transgenic Research Lab., Animal Biotechnology Division, NLRI, RDA.Suwon, Republic of Korea) | Park, J.K. (Transgenic Research Lab., Animal Biotechnology Division, NLRI, RDA.Suwon, Republic of Korea) | Kim, S.W. (Transgenic Research Lab., Animal Biotechnology Division, NLRI, RDA.Suwon, Republic of Korea) | Ko, E.M. (Transgenic Research Lab., Animal Biotechnology Division, NLRI, RDA.Suwon, Republic of Korea) | Kim, B.J. (Transgenic Research Lab., Animal Biotechnology Division, NLRI, RDA.Suwon, Republic of Korea) | Jo, S.J. (Transgenic Research Lab., Animal Biotechnology Division, NLRI, RDA.Suwon, Republic of Korea) | Byun, S.J. (Transgenic Research Lab., Animal Biotechnology Division, NLRI, RDA.Suwon, Republic of Korea) | Yang, B.S. (Transgenic Research Lab., Animal Biotechnology Division, NLRI, RDA.Suwon, Republic of Korea) | Chand, W.K. (Transgenic Research Lab., Animal Biotechnology Division, NLRI, RDA.Suwon, Republic of Korea) | Lee, H.T. (Konkuk University, Seoul, Republic of Korea) | Lee, P.Y. (Transgenic Research Lab., Animal Biotechnology Division, NLRI, RDA.Suwon, Republic of Korea), E-mail: [email protected]
This study was conducted to examine the effect of IRES controlled reporter gene on screening and production of recombinant human erythropoietin (EPO) proteins from cultured CHO cells. The cDNA was cloned for EPO from human liver cDNA, Using site-directed mutagenesis, we generated recombinant human EPO (rhEPO) with two additional N-glycosylations (Novel erythropoiesis-stimulating protein: NESP). Wild type hEPO and NESP were cloned into expression vectors with GFP reporter gene under regulatory control of CMV promoter and IRES so that the vectors could express both rhEPO and GFP.
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