Molecular Cloning and Characterization of the Gene for Outer Membrane Protein H in a Pasteurella multocida (D:4) Isolate from Pigs with Atrophic Rhinitis Symptoms in Korea
2004
Lee, J.M. (Sungkyunkwan University, Suwon, Republic of Korea) | Park, S.I. (Kyungwon University, Seoungnam, Republic of Korea) | Woo, H.J. (Seoul National University, Seoul, Republic of Korea) | Kang, S.Y. (Sungkyunkwan University, Suwon, Republic of Korea) | Kwon, M.S. (Sungkyunkwan University, Suwon, Republic of Korea), E-mail: [email protected]
A native strain of Pasteurella multocida was isolated from pigs suffering from severe atrophic rhinitis at domestic farms in Gyeonggi Province, Korea, and was identified as capsular serogroup "D" and somatic serotype "4" by disc diffusion decapsulation and gel diffusion precipitation tests, respectively. The P. multocida (D:4) induced atrophic rhinitis in healthy pigs by the secondary infection. The gene for outer membrane protein H (ompH) of P. multocida (D:4) was cloned in Escherichia coli DH5α by PCR.
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