Characterization and Expression Profile Analysis of a New cDNA Encoding Taxadiene Synthase from Taxus media
2005
Kai, Guoyin (Shanghai Jiao Tong University, Shanghai, P. R. China) | Zhang, Lei; Zhao, Dongli; Sun, Xiaofen (Fudan University, Shanghai, P. R. China) | Zhao, Lingxia (Shanghai Jiao Tong University, Shanghai, P. R. China) | Li, Zhugang (Shanghai Jiao Tong University, Shanghai, P. R. China) | Guo, Binhui (Shanghai Jiao Tong University, Shanghai, P. R. China) | Miao, Zhiqi (Shanghai Jiao Tong University, Shanghai, P. R. China) | Tang, Kexuan (Shanghai Jiao Tong University, Shanghai, P. R. China), E-mail: [email protected]
A full-length cDNA encoding taxadiene synthase (designated as TmTXS), which catalyzes the first committed step in the Taxol biosynthetic pathway, was isolated from young leaves of Taxus media by rapid amplification of cDNA ends (RACE). The full-length cDNA of TmTXS had a 2586 bp open reading frame (ORF) encoding a protein of 862 amino acid residues. The deduced protein had isoelectric point (pI) of 5.32 and a calculated molecular weight of about 98 kDa, similar to previously cloned diterpene cyclases from other Taxus species such as T. brevifolia and T. chinenisis. Sequence comparison analysis showed that TmTXS had high similarity with other members of terpene synthase family of plant origin.
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