Development of SCAR Markers for Early Identification of Cytoplasmic Male Sterility Genotype in Chili Pepper (Capsicum annuum L.)
2005
Kim, D.H. (Seoul National University, Seoul, Republic of Korea) | Kim, B.D. (Seoul National University, Seoul, Republic of Korea), E-mail: kimbd@snu.ac.kr
We previously used Southern blot analysis to detect restriction-length polymorphisms between male fertile and cytoplasmic male sterile (CMS) cytoplasms at the coxⅡ and atp6 loci of the mtDNA of Capsicum annuum L. Two copies of atp6 were found in each male fertile and CMS pepper lines. Interestingly, one of the copies of atp6 in CMS pepper was a 3'-truncated pseudogene. The open reading frame of the coxⅡ gene was the same in the fertile (N-) and CMS (S-) lines. However, the nucleotide sequence in the S-cytoplasm diverged from that in the N-cytoplasm 41 bp downstream of the stop codon. To develop CMS-specific sequence-characterized amplified region (SCAR) markers, inverse PCR was performed to characterize the nucleotide sequences of the 5' and 3' flanking regions of mitochondrial atp6 and coxⅡ from the cytoplasms of male fertile (N-) and CMS (S-) pepper plants.
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