Plant Regeneration through Somatic Embryogenesis of Leymus chinensis Trin.
2005
Kim, M.D. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) | Jin, Hua. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) | Park, E.J. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) | Kwon, S.Y. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) | Lee, H.S. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) | Kwak, S.S. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), E-mail: [email protected]
Chinese leymus (Leymus chinensis Trin.) is a perennial grass that is widely distributed at high pH sodic and arid soil in the northeastern Asia. An efficient regeneration system was established through somatic embryogenesis of mature seeds to understand its high adaptability to harsh environmental conditions on the basis of molecular biology. The calli were efficiently induced (about 70%) from mature seeds on MS medium supplemented with 1.5 mg/L 2,4-D. Somatic embryos were formed from the surface of embryogenic callus on MS medium supplemented with 2.0 mg/L kinetin and 0.5 mg/L NAA after 3 weeks of culture. Roots were induced from the shoot when transferred to MS medium without plant growth regulator for 1 week. Plant regeneration rate was 36% and regenerated plantlets were grown to normal mature plants in pot.
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