Affinity Immobilization of Dextransucrase on Dextran-based Support and the Production of Leucrose
2005
Han, N.S. (Chungbuk National University, Cheongju, Republic of Korea), E-mail: [email protected] | Kang, S.Y. (Chungbuk National University, Cheongju, Republic of Korea) | Lee, S.B. (Yonsei University, Seoul, Republic of Korea) | Robyt, John F. (Iowa State University, Ames, IA, USA)
A simple and convenient method of immobilizing dextransucrase via an affinity interaction is described, along with the use of this system to synthesize leucrose. Dextransucrase was produced in sucrose-free medium by fermenting a constitutive mutant of Leuconostoc mesenteroides NRRL B-512F and was separated using an ultrafiltration membrane. The purified enzyme was free of dextran polymer, which previously was always found with the sucrose-induced enzyme. Therefore, it was possible to immobilize the enzyme on dextran-based resins using an affinity interaction.
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