In vitro selection for salt tolerance among different strains ofBradyrhizobium japonicum
2003
Shereen, A. | Ansari, R. | Alam, S.M. | Mumtaz, S. | Raza, S.,Nuclear Inst. ofAgriculture, Tandojam (Pakistan)
An experiment was conducted to study the comparative salt tolerance of 12different strains of Bradyrhizobium japonicum (NIFTAL and USDA series) andstrains isolated from locally grown soybean nodules collected from 3 differentfield locations under different salinity levels of sodium chloride (0, 25, 50, 75and 100 mM NaCl corresponding to 0, 0.15, 0.29, 0.44 and 0.59 % NaCl,respectively). The rhizobium strains isolated from nodules were found to behighly sensitive when compared to the strains of NIFTAL and USDA seriessince there was a growth reduction of more than 80 % at the level of 50 mMNaCl. A wide range of variation was observed in the growth response ofNIFTAL and USDA series with comparatively less reduction at the level of 50mM NaCl. The strains TAL-950, TAL-102, TAL-103, TAL-377 were foundcomparatively salt tolerant.
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