Efficacy tests of antagonistic fungi and anthracnose pathogen resistant to chemical fungicide (carbendazim) for biological control of plant pathogen
1999
Yang Qian | Kasem Soytong | Veeranee Sripromsuk(King Mongkut's Institute of Technology Ladkrabang, Bangkok (Thailand). Faculty of Agricultural Technology. Dept. of Plant Pest Management Technology)
Antagonistic fungi, Chaetomium globosum Cg8, Ch. cupreum Cc9, Trichoderma harzianum T88-2 and transformant of T. harzianum China carrying resistant gene to carbendazim were tested to inhibit mango anthracnose caused by Colletotrichum gloeosporioides M002-2 in bi-culture antagonistic tests. Results showed that Ch. globosum and Ch. cupreum could resist to carbendazim at the concentration of 0.2 ppm which inhibited the mycelial growth and inoculum production of C. gloeosporioides as 60 and 87 percent. But for T. harzianum T88-2 showed the highest resistant to carbendazim at the concentration of 0.8 ppm which in bi-culture on PDA incorporated with carbendazim could inhibit the mycelial growth and inoculum of C. gloeosporioides as 90 and 89 percent, respectively. The transformant of T. harzianum carrying resistant gene to carbendazim which it is not effective bioligical control agent, it confirmed that the transformant could not inhibit the growth of C. gloeosporioides but it is only showed resistant to carbendazim at the concentration of 0.8 ppm or more.
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