Comparision of the in vivo digestibility with the in vitro digestibiligy for pigs
1999
Prapruek Tangmonkhong | Rattana Jungtongsin | Sinchai Paraksa | Nuanchan ParaksaKasetsart Univ. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture. Dept. of Animal Science)
To study the in vitro digestibility of protein and amino acids in six feedstuffs : broken rice, corn, rice bran, soybean meal, peanut meal and fish meal, the intestinal fluid collected from duodenum and ileum were incubated with each feedstuff in various times as following: 1) duodenal digesta for 8 hrs 2) duodenal digesta for 12 hrs 3) ileal digesta for 36 hrs and 4 ileal digesta for 48 hrs. The activity of each enzyme in duodenal and ileal digesta are : pepsin 5,000, 0 trypsin 94500, 58500 and chymotrypsin 3100, 2500 units per milliliter, respectively. By using factorial analysis now that type of feedstuff effected on the coefficient of digestibility significantly, whereas type of digesta and the incubation time only effected on the digestibility of dry matter and some amino acids such as, tryptophan, isoleucine, lysine and phenylalanine. For broken rice, soybean meal, peanut meal and fish meal, the duodenal digesta provided the better results than the ileal digesta and 12 hrs-incubation show the higher digestibility than 8-hrs-incubation. Contradictory, feedstuff containing high fiber content such as rice bran and corn, incubation with ileal digesta show the superior digestibility than duodenal digesta and incubation time for 36 hrs is better than 48 hrs. The in vitro digestibility of protein and amino acids by incubation with duodenal digesta show the significantly correlation with the in vivo apparent ileal digestibility and incubation for 12 hrs show higher value than 8 hrs. In the same way, the correlation between the in vivo total digestibility of protein and amino acids and the in vitro digestibility incubated for 36 hrs with ileal digesta is higher than that incubated for 48 hrs.
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