Effects of sodium phosphate and potassium di hydrogen phosphate ratio on growth of Chaetoceros calcitrans, Paulsen
2000
Sathit Kowitwati(Rajabhat Institute Bansomdejchaopraya, Bangkok (Thailand). Faculty of Science and Technology)
Experiment on the use of 5 phosphates, namely Na2HPO4, Na2HPO4.2H2O, Na2HPO4.12H2O, K2HPO4 and NaH2PO4.H2O to replace the phosphate f/2 culturing formulation at the same concentration in 1 liter of sea water at 30 ppt with pH equal to 8.10. The initial culturing of diatom was at density of 5*10**(4) cells per milliliter and the light source from fluorescence tube at light intensity of 570 lux and air bubbles given throughout the experiment. It was found that Na2HPO4.12H2O and K2HPO4 phosphate gave the maximum number of diatom in the 7th day which was equal to 295.00*10**(4) and 224.64*10**(4) cells per milliliter, respectively. Number of both cells was highly significant difference (P LT 0.01). When both 2 kinds of phosphates, namely K2HPO4 and Na2HPO4.12H2O at the ratio of 1:0, 1:3, 1:1, 3:1 and 0:1 were used for culturing diatom. It was found that the ratio of 3:1 provided the maximum number of diatom cells (368.26*10**(4) cells per milliliter). In the 6th days of culturing there was the division rate (k) from day 0 to 5 equal to 1.1213 with the regression relationship between average number of diatom and culturing day as number of diatom=239260.28-869077.47 day+430432.40 days**(2)-34669.85 day**(3), r sup(2)=0.9414.
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