Investigations on possibilities of biological control of Scterotima Stem rot of rapeseed by antagonistic microorganisms Invitro and inviro conditions
2003
Afshri Azad, Homayon | Naseri, Bita | Ershad, Jafar
White stem rot is one of the important diseases of rapeseed specially in northen Iran. Biological control is one of the means for the management of this disease. To achieve this goal, during a 2 years survey, 40 antagonist isolates comprising 19 bacterial and 21 fungal isolates were isolated from rapeseed in Mazandaran, Golestan and Zanjan provinces and were evaluated in invitro condition. Antagonistic efficacy of these isolates were evaluated on the basis of inhibition of fungal growth, rate of fungal growth, sporulation of fungus in different culture media, different temperature and lysis of sclerotia. Nine bacterial and one fungal isolates were selected for greenhouse studies. Virulence of 6 isolates collected from different hosts and locations were tested on rapeseed plant, and SK1 was the isolate selected for greenhouse studies. The results of this experiment revealed that benomyl in both treatments (sequesntial and concomitant), and concomitant inoculation of B4, B14 and Tr2 with the disease causal agent showed the best effect on the control. However, B6 and B7 isolates showed better effect in sequential inoculation (i.e. inoculation of antagonist before the disease causal agent). No interaction was observed among position of leaves and other factors (time of inoculation and other control agents).
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