Hybridiziation between diploid and tetraploid cotton species and investigation on hybrids
2003
Alishah, Omran | Aghdasi, Mahnaz | Parichehre, Ahmadian
Cultivated cotton include four diploid and tetraploid speciesG. hirsutum (2n= 4x= 52) with high yield, G. barbadense( 2n= 4x= 52), with high quality G. herbaceum (2n= 2x= 26) and G. arboreum (2n= 2x= 26) with environmental stress tolerance. Interspecific hybridization can be used in gene transfer, hybrid production, development of euploids and aneuploids and study of phylogenic relationships. Diploid and tetraploid species are incompatible and embryo rescue have to be employed for hybridization. In order to optimize this technique for making crosses among different species and to find the response of embryo to various media, all possible crosses among four cultivated cotton species were made. The results ofembryos of hybrids and parents were cultured on four different media. The analysis of data on the base of factorial experiment in randomized complete block design showed that in- ovulo embryo culture technique is more suitable for embryo rescue in cotton. The results also showed that genotype , media and the their interaction affect on callus size, percentage and the growth rate of in -ovulo embryo. G. herbaceum, G. arboreum and G. hirsutum had more callogenesis, while G. barbadense and its hybrids demonstrated higher germination and regeneration, suggesting G. barbadense can be a useful donor parent and also MS3 being a suitable media.
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