Search for mutant genes in the cultured green-type mutant strain of Porphyra yezoensis
2006
Mizukami, Y.(National Fisheries Univ., Shimonoseki, Yamaguchi (Japan)) | ; Kawasaki, T. | ; Murase, N. | ; Kunimoto, M.
The green-type pigmentation mutant strain of Porphyra yezoensis was examined for the genomic genes related to the pigmentation mutation. Although this strain is a pigmentation variant, the nucleotide sequences of six phycobilin-related genes and ribulose bisphosphate carboxylase-oxygenase (Rubisco) coding regions did not vary between the wild and mutant-type strains. The RAPD analysis of the total genomic DNA showed 80.1 % similarity of DNA fragments between these two strain types in the electrophoretic mobility. From DNA fragments specific for the wild or mutant strain in the RAPD pattern, eight fragments (eight loci) were chosen arbitrarily for the analysis of nucleotide sequences. Three of the fragments consisted of nucleotide sequences similar to those of retrotransposons in higher plants. For other five fragments, no corresponding gene was specified by the gene bank searches.
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