Electro protoplast fusion between Solanum sisymbriifolium and other Solanum species
2006
Oda, N.(Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture) | Isshiki, S. | Sadohara, T. | Ozaki, Y. | Okubo, H.
Symmetric or asymmetric protoplast fusions between Solanum sisymbriifolium and other Solanum species (S. integrifolium and S. toxicarium) were performed to obtain the perfectly soil-born disease resistant rootstocks for eggplant cultivation. Somatic hybrid was not obtained from symmetric fusion in selective media, where one parent's protoplasts formed colonies and the other did not. Combining of iodoacetamide-induced inactivation of S. integrifolium and no cell division ability of S. sisymbriifolium on a selective medium in asymmetric fusion, the hybridity of two calli was elucidated by analyzing IDH, PGM and SKDH isozymes. Plant regeneration from the somatic hybrid calli was also successful, but the regenerated plants did not show the hybridity. The result may be caused by the elimination of S. integrifolium chromosomes during the process of shoot regeneration from calli.
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