Effect of Fasciola gigantica excretory secretory antigen on rat hematological indices
2006
Ganga, G. (Indian Veterinary Research Institute, Izatnagar, Bareilly, India) | Varshney, J.P. (Indian Veterinary Research Institute, Izatnagar, Bareilly, India), E-mail: [email protected] | Sharma, R.L. (Indian Veterinary Research Institute, Izatnagar, Bareilly, India)
The present study was undertaken to investigate the effect of Fasciola gigantica excretory secretory antigen (Fg-ESA) on rat hematological indices. Fg-ESA was prepared by keeping thoroughly washed 40 F. gigantica flukes in 100 ml phosphate buffer saline (PBS) for 2 h at 37℃, and centrifuging the supernatant at 12,000 g at 4℃ for 30 min. The protein content of Fg-ESA was adjusted to 1.8 mg/ml. The rats were randomly divided into two groups of six rats each. Rats in group A received 0.5 ml of Fg-ESA intraperitoneally (i.p.) for 7 days, whereas control rats in group B received 0.5 ml of PBS i.p. for 7 days.
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