The changes of the concentrations of thiamin and riboflavin in milk enriched with prebiotics and probiotics
2006
Beitane, I. | Ciprovica, I.
Fermentation of milk with probiotics and prebiotics has been shown to increase the concentration of B group vitamins in fermented milk products. The task of the study was to investigate the influence of prebiotics on the increasing of thiamin and riboflavin concentrations in milk during fermentation by Bifidobacterium lactis. Lactulose (Duphilac, Netherlands) and inulin (Raftiline, Belgium) were added (1, 2, 3, 4 and 5%) into 100 g of milk. The milk samples were inoculated with Bifidobacterium lactis (BB-12, Chr. Hansen, Denmark) and incubated at 38 deg C for 16 hours. The amount of thiamine and riboflavin was determined by fluorometric method. Laboratory studies have shown that the increasing of thiamin and riboflavin concentrations in milk depends on the concentration of lactulose and inulin. Data on the final thiamin and riboflavin concentrations in fermented milk indicates that increasing the lactulose concentration from 1% to 3% enhanced the production of thiamin and riboflavin. The similar data was obtained with inulin. Increasing of inulin concentration from 1% to 4% enhanced the production of vitamins thiamin and riboflavin. The present results furthermore indicate that finding optimal combination of prebiotics and probiotic pairs where the prebiotic would benefit the specific probiotic strain, e.g. during production and formulation into foods, is one of the research priority for functional foods.
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