Response of pea plants (Pisum sativum L.) to reduced supply with molybdenum and copper
2006
Hristozkova, M. (Bulgarian Academy of Sciences, Sofia (Bulgaria). Dept. of Mineral Nutrition and Water Regime) | Geneva, M. | Stancheva, I.
The role of molybdenum (Mo) and copper (Cu) in regulation the activities of the enzymes involved in primary nitrogen assimilation in particular the nitrate reductase (EC 1.6.6.1) and glutamine synthetase (EC 6.3.1.2) was examined. Pea plants were grown in a phytotron chamber at 12 h photoperiod, day/night temperature 25/18 degree C and photon flux density of 95 mumol m-1 s-1 until 21-st day. Plants were grown at full strength Helriegel nutrient solution competed with micronutrients as in Hoagland and Amon and reduced Mo and Cu concentrations. Although only extremely small amounts of Mo and Cu are required for normal plant growth, reduced supply with Mo and Cu to the growth medium decreased activities of the enzymes (nitrate reductase and glutamine synthetase) involved at initial steps of nitrate assimilation, fresh weight, and plastid pigment content (total chlorophyll and carotenoids). Accumulation of nitrates in plant tissues enhanced, especially in the varian ts with restrictive Cu concentration.
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