Exploring vertical and horizontal gene transfer in Pasteurella multocida PM70 by comparative genomics studies
2007
Hussain, M. (Karachi Univ. (Pakistan). Inst. of Biotechnology and Genetic Engineering) | Khan, M.T. (Karachi Univ. (Pakistan). Inst. of Biotechnology and Genetic Engineering) | Wajid, A. (Karachi Univ. (Pakistan). Inst. of Biotechnology and Genetic Engineering)
The present study has been conducted to explore different patterns and modes of Pasteurella multocida evolution. Total protein of P. multocida Pm70 with genomic synteny were subjected to total best protein identity comparison to all sequenced microbes at cutoff value of identity 70%; similarity 70% and p-value. Proteome identity of test and closely matched organisms lies in the range of 23.9% to 0.72%. The findings were further analyzed by comparative 16sr DNA sequence alignment. Entire nodal branch, leading to P. multocida was selected for further analysis. Closest relatives thus found belongs to the species of genera Mannheimia and Haemophilus which were compared for C-Level paradox, AT content, purine stretches, simple repeats, gene density, origin and terminal of replication etc. Fourier transformation was applied to transform entire DNA of test and phylogenetically related microbes into numerical values. From mentioned digitization, stack ing energy, protein deformability, propeller twists were deduced; this provided conspicuous evidences of potential vertical and lateral gene transfer in P. multocida. Additionally, similarity in the pattern of pseudo 2D gel profile among the microbes present in targeted nodal branch distinguished between physicochemical nature of horizontally and vertically transferred genes.
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