Bacterial population on seed of pepper and methods for identification [of] Xanthomonas campestris pv. vesicatoria. [Master thesis]
2006
Delibasic, T.
Under the conditions of modern vegetable production bacterial diseases are significantly important from economical point of view. Xanthomonas campestris pv. vesicatoria cause the most losses on pepper. The most important source of primary inoculum for bacterial diseases is seed. Very low percent infection of seed as 0.1%-0.01% are able to cause epidemic in convenient conditions. Detection of bacterium X. c. pv. vesicatoria on the pepper seed was investigated during 2002-2004. Direct and indirect isolation methods were applied. Using direct methods, on commercial seed of pepper, does not prove presence of phytopathogenic bacteria. It is prove only presence mycelium of saprophytic fungi and Fusarium sp. Reliable positive and repetable results were obtained in condition of artificial inoculation of seed and isolation from seed extract to special selective media. The most successful selective media for X. c. pv. vesicatoria isolations from seeds were media according by McGuire and Jones (1984, 1989). Investigated samples of commercial pepper seed showed that these samples are free of bacterium X. c. pv. vesicatoria.
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