The high-performance liquid chromatographic determination of sulphadimidine residues in eggs
2004
Kozarova, I.,University of Veterinary Medicine, Kosice (Slovak Republic) | Hussein, K. | Mate, D. | Jevinova, P.
Sulphadimidine is widely used for the treatment of coccidiosis in laying hens at a dose of 2 g.l-1 for six consecutive days via drinking water. As a result, there is concern that the residues of this drug may be retained in the eggs and present a potential risk to human health. The aim of our study was to determine the residue concentra-tions of sulphadimidine in the whole eggs of laying hens during and after its oral administration up to the 15th day of the withdrawal period. Sulphadimidine residues were determined by a gradient high-performance liquid chromato-graphy system with a photo-diode array detector at 275 nm. Our resulth indicate that the administration of sulpha-dimidine to the laying hens produced a rapid and sustained increase in sulphadimidine residues in eggs. The highest residue concentrations of sulphadimidine were found on the sixth day of its oral administration. After withdrawal, the residues declined rapidly, but they were still detectable on the tenth day of the withdrawal period. On day 11, the residues were below the established maximum residue limit (MRL) (0.1 mg.kg-1). The limit of quantification (LOQ) for sulphadimidine was 0.3 mg.kg-1,the detection limit (LOD)was 0.09 mg.kg-1, and the recovery range from 91 to 98%.
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