Detection of hybrids in commercially propagated Eucalyptus using 5S rDNA sequence
2003
Steenkamp, E.T.,University of York, York (United Kingdom). Department of Biology | Nest, M.A. van der,University of Pretoria, Pretoria (South Africa). Department of Genetics | Wingfield, M.J.,University of Pretoria, Pretoria (South Africa). Department of Genetics | Wingfield, B.D.,University of Pretoria, Pretoria (South Africa). Department of Genetics
In South Africa, Eucalyptus grandis has been used extensively in commercial plantations. E. grandis, together with two other important South African forestry species (E. saligna and E. urophylla) form part of the Eucalyptus section Transversaria. Identification of these species and their hybrids is complicated by the presence of overlapping morphological features. Application of molecular diagnostics would, therefore, greatly simplify routine verification of species and hybrid identity. Our aim was to assess the value of the 5S ribosomal DNA repeat, for developing a PCR-based diagnostic method for differentiating E. urophylla, E. saligna and E. grandis. For this purpose we analysed the 5S repeat from E. urophylla, E. saligna and E. grandis, grown from seed that was collected from native trees known to represent these species in Australia and Indonesia. Three commercially grown E. grandis individuals were also included. Sequence analysis showed that the 5S spacer is sufficiently variable for distinguishing E. grandis, E. urophylla and E. saligna from each other. It also revealed that two of the three commercially grown E. grandis in South Africa are hybrids. Despite the utility of this approach the variable sites in the Eucalyptus 5S repeat appear to be unsuitable for constructing species-specific PCR-primers.
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