The seroprevalence of Ehrlichia canis in dogs in East Slovakia
2004
Letkova, V.,University of Veterinary Medicine, Kosice (Slovak Republic) | Mojzisova, J. | Winkler, R. | Curlik, J. | Bajova, V.
The etiologic agent of canine monocytic ehrlichiosis (CME) is the rickettsia Ehrlichia canis.They are small Gram-nega-tive coccoid bacteria that are parasitic on circulating monocytes in clusters of organisms, called morulae. E.canis is transmitted by the brown dog-tick Rhipicephalus sangui-neus.The distribution of CME is related to the distribution of the vector and has been reported occuring in Asia,Africa, Europe and America.To date no case reports of CME have been documented in Slovakia. This study is to find out the sero-prevalence of E.canis in dogs in Slovakia. Serum samples from 78 dogs were collected from different parts in Eastern Slovakia, and were tested by an indirect immunofluorescence technique (IFA) and by ELISA Immunocomb analysis (Biogal Kibbutz Galed Israel). On the IFA analysis 29 dogs (37.2 %) reacted to E. canis antigens (with a titer 1:64). According ELISA Immunocomb analysis (Biogal Kibbutz Galed Israel) positive results are expressed in scales units 1-6; degree 3 is consideret as positive.Sixteen dogs }55.2%) had anti-body titers of 'S'units 3, eleven dogs (37.9%) had anti-body titers of 'S' units
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