Embryogenesis in durian
2001
Charuwan Chatisathian(Department of Agriculture, Bangkok (Thailand). Biotechnology and Nuclear Technique Office) | Suphap Suntharanon(Department of Agriculture, Bangkok (Thailand). Horticulture Research Institute. Chantaburi Horticulture Research Center)
Embryogenic callus can be induce from ovule seeds of Durio zibethinus Murr., variety Montong. The appropriate fruit ages were 8-20 days or 1-3 weeks after anthesis. The older and the younger ones were not induced embryogenic callus. Callus can be obtain from 2 different kinds of induction media, one has been Murashige and Trucker basal medium with 500 mg/l malt extract, malt extract, 0.1 mg/l IAA, 1 mg/l kinetin(Nu5) and another one has been Murashige and Skoog basal medium with 1 mg/l NAA(MSN). Subculture every 3 weeks can be reduced the phenolic compound productions and obtained to maximum of embryogenic callus proliferation. This technique also was suitable for embryogenic callus induction in Durio giffithii (Mast.) Bakh., the wild type of durian in Thailand.
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