Lipase-producing microorganisms for use in kitchen wastewater treatment: [Final research report: Kasetsart University research fund fiscal year 2002]
2002
Orapin Bhumibhamon(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Agro-Industry. Department of Biotechnology | Kriangkrai PhattayakornKasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Agro-Industry. Department of Biotechnology)
Results showed that, at shaker 250 rpm, fat was highest degraded 55.91 percent and 60.42 percent in pure cultures of Pseudomonas sp. (KLB1) and mixed cultures respectively. The studies of inoculum size of pure and mixed cultures were 1-2.5 percent and 5 percent which have the highest efficiency of fat degradation (61.78-65.19 percent and 60.42 percent). Initial COD 4,000 mg/l has the highest efficiency in fat and COD degradation (83.46 percent and 95.81 percent). Results showed that the highest efficiency of aerobe conditions in fat degradation and lipase activity after added 8 percent cultures in period (50.02 percent and 0.76 U/ml). Moreover after adding 8 percent Pseudomonas sp. (KLB1) lipase activity in aerobe conditions in tank (domestic kitchen wastewater) was detected. After that lipase activity was reduced and finally absence. Thus, the immobilization of microorganism cells on plastic balls has been used. Results showed that cells immobilization could increase efficiency of wastewater treatment in tank. These results showed that lipase producing microorganisms could degraded fat in tank. Thus, microorganisms could applied in domestic or industrial wastewater treatment system in order to fat increasing efficient of systems by reduce to block of fat.
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