Induction of doubled haploids in maize by anther culture: [Final research report: Kasetsart University research fund fiscal year 1997]
1997
Pittayaporn Boonyai(Kasetsart University, Bangkok (Thailand). Inseechandrasatitya Institute for Crop Research. Suwan Wajokkasikit Field Crops Research Station | Nitsri SangduenKasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Science. Department of Genetics | Prapa SripichittKasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Agriculture. Department of Agronomy)
Eight Thai hybrid varieties maize were tested for their response to the process of induction of embryo like structure (ES). The result showed that seven Thai hybrids varieties maize from the eight tested hybrids varieties responded to the process of ES induction in the range of 0.15-7.02 percent, however, there were only three hybrids varieties responded to plantlet regeneration in the range of 8.75-22.2 percent. The results of the present study also revealed that the anther of the F1 plant of the cross between the eight tested hybrids varieties and maize variety from Switzerland (M22/4/1) remarkable improved in response to ES induction and plantlet regeneration. In another word, the response to ES induction increased in every cross to the range of 4.05-50.06 percent , and the response to plantlet regeneration increased to the range of 7.43-29.41 percent. During transplanting stages, the most founded causes to the mortality of the plantlets in growth mrdia (GM), were retarded growth on shoot and root, shoot wilting due to hardening process as well as unexpanding young leaves. However, transplanting plantlets from GM to Hoagland solution resulted in 46.05 percent of survival which was much higher, compared to 21.05 percent of survival when the plantlets were transplanting in to the soil. The most common caused of mortality founded in the plantlets in Hoagland solution were wilting of shoot, no forming of new root as well as root rotting. Whilst the common causes of mortality to plantlets in the soil were rotting of stem base, root and shoot wilting.
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