A modified low copy number binary vector pUN for Agrobacterium-mediated plant transformation
2007
Vaculkova, E.,Slovenska Akademia Vied, Nitra (Slovak Republic). Ustav Genetiky a Biotechnologii Rastlin | Moravcikova, J.,Slovenska Akademia Vied, Nitra (Slovak Republic). Ustav Genetiky a Biotechnologii Rastlin | Matusikova, I.,Slovenska Akademia Vied, Nitra (Slovak Republic). Ustav Genetiky a Biotechnologii Rastlin | Bauer, M.,Vyskumny Ustav Zivocisnej Vyroby, Nitra (Slovak Republic) | Libantova, J.,Slovenska Akademia Vied, Nitra (Slovak Republic). Ustav Genetiky a Biotechnologii Rastlin
A modified low copy number plant binary vector pUN has been constructed and successfully used to clone unstable DNA sequences. The vector pUN comprises of low copy number, broad host range RK2 replicon from pBin19 and of multiple cloning site (MCS) and T-DNA region, both from a pBINPLUS-derived pLV06 vector. The absence of the ColE1 replicon in the backbone of the binary vector significantly contributed to the stability of hardly clonable DNA sequences and enabled their transfer into the tobacco plants through Agrobacterium-mediated transformation.
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