Chitinase characterization from Clostridium bifermentans Sp2-1
2004
Meryandini, A.((Institut Pertanian Bogor (Indonesia). Fakultas Matematika dan Ilmu Pengetahuan Alam))
Chitinolytic bacterium, Clostridium SP 2-1, was isolated from slaughtered house Segara Alas, 16S rRNA sequence analysis indicated that Clostridium was closely related to C. bifermentans. This isolate produced maximum chitinase activity after 24 hours of cultivation in liquid media. The chitinase displayed maximum hydrolitic activity for colloidal chitin at 60 degree C and pH 7.5. Divalent ions such as Fe2+, Cu2+, Co2+, Zn2+, Ca2+ at 1 mM concentration increased enzyme activity, while at the same concentration Mg2+ and Mn2+ inhibited chitinase activity. At higher concentration of 5 mM all ion were inhibitory. The presence of Na2EDTA at 1 mM and 5 mM inhibited enzyme activity.
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