Semi Photoautotrophic Micropropagation On Three Eucalyptus Species
2007
Assareh, M.H., Research Institute of Forest and Rangelands, Tehran, Iran, | Vatanpour, Z., Al-Zahra University, Tehran, Iran. | Kiarostami, Kh., Al-Zahra University, Tehran, Iran. | Ghamari Zare, A., Research Institute of Forest and Rangelands, Tehran, Iran.
Eucalyptus trees are fast-growing trees and some of species are tolerance to un-favorite conditions. Seedling production is a pre-condition for agroforestry in suitable regions of Iran. Micropropagation is a useful tool for asexaul production. Conventional micropropagation is an expensive method, because of high rate contamination and death of plantlets during transferring to soil and using high amount of biochemical plant growth regulaturs. Semiphotoautotrophic system is used because of omitting sucrose in the media, using exogenesis growth regulators in low amount or not using at all, and better rooting and shooting of plantlets. So this system decrease the expenses of in vitro plantlets production. This work, was studied the effects of G7 and B.F.J with and without filtered cap. Growth indicators,i.e. root fresh yield, root dry yield, shoot fresh yield, shoot dry yield, leaf surface, leaf number, root length, shoot length, and shoot number, of three difference species Eucalyptus camaldulensis, E. melliodora and E. micrpcarpa were survayed in semiphotoautotrophic conditions.The G7 filtered cap vessels were better than the other for growth indicators which were studied. The range of survival for transferring seedling to soil was 100% for E. melliodora and E. micrpcarpa species and 65% for E. camaldulensis. Semiphotautotrophic not only reduce the micropropagation costs, but also improve the growth indicators. However, its problems, i.e. surface and internal contamination of explants, phenolic components release and etc. should be considered in semephotoautotrophic micropropagation system.
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