Biological and serological comparison of tospoviruses isolated from ornamental of Tehran and Markazi provinces

To study serological and biological differences of tospoviruses that infect ornamentals of Tehran and Markazi provinces, ornamental plants which seems to be infected by viruses were tested using polyclonal antibody in DAS-ELISA against tomato Varamin virus (ToVV) (AS-0526), iris yellow spot virus (IYSV) (AS-0528, AP-028) and by monoclonal antibody (Mab) in TAS-ELISA against tomato spotted wilt virus (TSWV) (AS-0106/0116, AS-o105), impatiens necrotic spot virus (INSV) (AS-0117, AS-011115) which were gifted by DSMZ-Braunschweig- Germany. Tospovirus infected positive samples were then triturated in chilled 01M potassium phosphate buffer pH=7 containing 0.15%, 2-mercaptoethanol. Sap inoculated on indicator hosts: Nicotiana bentaminana, N.rustica, N.tabacum cv. Samsum, vigna uniguiculata, Datura metel, D.srtamonium, Lycopersicon esculentum, Petunia hybrida and Chenopodium amaranticolor. The local symptoms were observed noted after 5 days and the systemic symptoms appeared approximately after 20 days: TSWV infected plants produced necrotic local lesion and necrotic ring spot followed by, mosaic and leaf deformation symptoms on N.bemthamiana, sever necrotic local lesion, chlorotic and mosaic on N.tabacum cv. Samsun, sever necrotic and necrotic ring spot, leaf deformation and mild local lesion necrotic on Vigna unguicolata, sever necrotic local lesion and necrotic ring spot, leaf deformation on Datura metel, sever necrotic local lesion, necrotic ring spot, leaf deformation, mosaic and death of total plant on D.stramonium, necrotic local symptoms on Petunia hybrida, necrotic local and mosaic on Lycopersicon sculentum cv. Avand, necrotic local on Chenopodium amaranticolor. ToVV produced systemic leaf deformation on N.benthamiana, necrotic local, systemic leaf deformation N.rustica systemic, leaf deformation on N.tabacum cv. Samsun, mild necrotic local on V.unguiculata, local clorotic lesion and ring spot local, systemic leaf deformation on D.metel systemic, leaf deformation on D.stramonium, local necrotic lesion on Petunia hybrida, necrotic local, mosaic on L.esculentum, necrotic local lesion on Ch.amaranticolor. IYSV produced leaf deformation on N.benthamiana, mild local necrotic lesion, necrotic systemic ring spot on V.unguiculata, necrotic local on P.hybrida INSV produced, necrotic local lesion, systemic leaf deformation, chlorosis and death of plant on N.benthamiana, Chlorotic and ring spot, leaf deformation and mosaic on D.metel, local chlorotic and ring spot, systemic chlorosis and mosaic on D.stramonium, necrotic local and systemic necrotic ring spot symptoms on Ch.amaranticolor. In addition to serological differences in tospoviruses isolated from ornamentals, there are also biological and host range symptoms differences noted which can be use preliminary to study these tospoviruses in greenhouse condition.