Molecular sexing in Java sparrow (Lonchura oryzivora)
2006
Ichikawa, A.(Aichi-ken. Agricultural Research Center, Nagakute (Japan)) | Ichimura, T. | Nakamura, A. | Noda, K.. | Kato, Y.
Sex identification based on morphological characters is not possible in Java sparrow ( Lonchura oryzivora ). Many molecular methods have been reported to identify the sex for many species of birds including chicken. In the present study, seven PCR-based sexing protocols, involving two pairs of primers designed to amplify the fragment of the chicken W-chromosome specific repetitive DNA sequence Xho region and five pairs of primers to amplify the chromo-helicase-DNA (CHD) gene that is located on both the avian Z- and W-chromosomes, were applied to Java sparrow. The sex of Java sparrow could be identified by a PCR assay with 3 primers of CHD gene (2945F-cfR-3224R, Ellegren 1996), which were designed from the sequence of CHD-W gene of collared flycatcher. No fragment or no female specific fragment was detected from PCR amplification using the other pairs of primers. Furthermore, blood sampling from the claw was attempted to develop an easy blood sampling method with minimal damage in Java sparrow. Using this method, we could get enough amount of DNA sample from individuals over the age of 4 weeks.
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