Changes in the gene expression of adiponectin and glucose transporter 12 (GLUT12) in lactating and non-lactating cows
2007
Komatsu, T.(National Inst. of Livestock and Grassland Science, Tsukuba, Ibaraki (Japan)) | Itoh, F. | Sakumoto, R. | Hodate, K. | Obara, Y. | Kushibiki, S.
Glucose delivery and uptake by the mammary gland is a rate-limiting step in milk synthesis. Insulin resistance is believed to increase throughout the body following the onset of lactation. To study glucose metabolism in peak-, fate-, and non-lactating cows we analyzed the expression of an adipokine, namely, adiponectin, decreased insulin resistance, leptin, and a novel insulin-responsive glucose transporter (GLUT12) in the adipose tissue and mammary gland by using real-time polymerase chain reaction. Our results demonstrated that the mRNA level of adiponectin in the adipose tissue was greater in non-lactating cows than in peak-lactating cows. In the adipose tissue, there were no significant differences in the abundance of GLUT12 mRNA between the peak-, late-, and non-lactating cows. In contrast, in the mammary gland, the mRNA level of GLUT12 was greater in non-lactating cows than in peak- and late-lactating cows. In the adipose tissue, the mRNA level of leptin and peroxisome proliferator-activated receptor gamma 2 (PPARgamma2) was greater in non-lactating cows than in peak-lactating cows. The results of the present study suggest that in lactating cows adiponectin plays an important role in insulin resistance in the adipose tissue; in the mammary gland, GLUT12 expression is believed to be an important factor for insulin-dependent glucose metabolism.
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por The Agriculture, Forestry and Fisheries Research Information Technology Center