Analysis of genetic resource in Zoysia spp, 2: Evaluation of genetic diversity in zoysiagrass indigenous to southwest islands of Japan based on simple sequence repeat markers
2007
Hashiguchi, M.(Miyazaki Univ. (Japan). Frontier Science Research Center) | Tsuruta, S. | Matsuo, T. | Ebina, M. | Kobayashi, M. | Akamine, H. | Akashi, R.
We conducted a study of genetic diversity in zoysiagrass indigenous to Southwest Islands of Japan based on simple sequence repeat (SSR) markers. Forty-one lines of zoysiagrass, which included 19 Zoysia matrella, 18 Z. tenuifolia and 4 cultivars (Asagake: Z. japonica, Emerald: Z. japonica x Z. tenuifolia, Tottori-korai: Z. matrella, Velvet: Z. tenuifolia), were used in this study. From 12 SSR markers used, a total of 155 SSR bands were scored. The number of putative alleles ranged from 6 to 22, with average value of 12.9. The polymorphic information contents (PIC) of Z. matrella and Z. tenuifolia were 0.79 and 0.72, respectively. Z. matrella had significantly higher PIC than Z. tenuifolia (p= 0.02). Cluster analysis based on the 155 SSR bands revealed that the 41 lines were classified into 7 groups.
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