Efficient rooting and root development after transfer of regenerated plantlets of Camptotheca acuminate
2007
Wang, H.(Northeast Forestry Univ., Harbin (China)) | Zu, Y. | Liu, H.
For establishing an efficient in vitro rooting protocol of Camptotheca acuminata and improving the survival rate and acclimatization of the regenerated plantlets, the effects of auxins, sucrose concentration and different basal medium on in vitro rooting and root development of regenerated shoots after transfer were investigated in this study. Auxin played an important role in in vitro rooting and rooting ability was affected by auxin type and the concentration. IBA (indolebutyric acid) 0.5 mg/l was the most effective, providing high shoot regeneration frequency (98%) as well as the highest number of roots (5.9 roots per shoot). Sucrose concentration from 10 g/l to 30 g/l was positively correlated with the rooting percentage and root number, however, higher sucrose concentration (40 g/l) negatively inhibited rooting. WPM or 1/2MS medium produced better results than MS medium. Optimum rooting was attained on WPM or 1/2MS medium supplemented with IBA 0.5 mg/l, sucrose 30 g/l and agar 6 g/l. 96% of the in vitro rooted plantlets with well developed shoots and roots survived after transfer. Root development of regenerated plantlets was much worse than that of seedlings after transfer. The ratio between root tips and leaf area in regenerated plantlets were significant lower than that of seedlings.
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