Sequence analysis of VP2 gene of infectious bursal disease virus field isolate and vaccine strains
2006
Jin, J.D. (Kangwon National University, Chuncheon, Republic of Korea) | Kang, Z.W. (Kangwon National University, Chuncheon, Republic of Korea) | Kim, S.J. (Seoul National University, Seoul, Republic of Korea) | Kwon, H.M. (Kangwon National University, Chuncheon, Republic of Korea) | Hahn, T.W. (Kangwon National University, Chuncheon, Republic of Korea), E-mail: twhahn@kangwon.ac.kr
The VP2 full gene of Korean infectious bursal disease virus (IBDV) strain, SH/92, three attenuated vaccine strains, Bur706, Bursine-2 and CEVAC strains, were amplified by reverse transcriptase-polymerase chain reaction and sequenced and compared with published PV2 gene sequences of IBDVs. The VP2 nucleotide sequence similarity between SH/92 and three vaccine stains was 95.6~96.5% whereas the nucleic acid similarity among three vaccine strains was 97.5~98.5%. The amino acid sequence similarity of VP2 of SH/92 compared with three vaccine strains was between 94.4 and 97.6% while the amino acid similarity among three vaccine strains was between 97.4 and 98.4%.
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