Effects of Heavy Metals on the in vitro Follicular Steroidogenesis in Amphibians
2006
Choi, M.J. (Chonnam National University, Gwangju, Republic of Korea) | Ahn, R.S. (Pochon CHA University, Seoul, Republic of Korea) | Kwon, H.B. (Chonnam National University, Gwangju, Republic of Korea), E-mail: [email protected]
Heavy metals are well known as important environmental pollutants and also considered as endocrine disrupters. This study was performed to evaluate the direct effects of heavy metals such as cadmium (Cd), zinc (Zn), mercury (Hg), lead (Pb), cobalt (Co), and arsenic (As) on the various steroidogenic enzymes in frog ovarian follicles. Ovarian follicles from Rana catesbeiana were isolated and cultured for 18 hours in the presence of frog pituitary homogenate (FPH, 0.05 gland/ml) or various steroid precursors with or without heavy metals (0.01-100 μM), and steroid levels in the follicle or culture medium were measured by radioimmunoassay (RIA).
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