A Molecular Sex Identification Using Duplex PCR Method for SRY and ZFX-ZFY Genes in Red Deer and Elk
2007
Han, S.H. (National Institute of Subtropical Agriculture, RDA, Jeju, Republic of Korea) | Lee, S.S. (National Institute of Subtropical Agriculture, RDA, Jeju, Republic of Korea) | Ko, M.S. (National Institute of Subtropical Agriculture, RDA, Jeju, Republic of Korea) | Cho, I.C. (National Institute of Subtropical Agriculture, RDA, Jeju, Republic of Korea), E-mail: [email protected]
This study was focused on discriminating the molecular sexes of red deer and elk by duplex polymerase chain reaction(PCR) using two primer sets. Sex differentiation of mammals is primarily dependent on the presence or absence of sex determining region Y(SRY) gene encoded on Y chromosome which plays a key role for male development. Zinc finger X-Y(ZFX-ZFY) gene, one of X-Y homology gene group was found on X- and Y- chromosomes, respectively. At first, the nucleotide sequences were characterized for the intron 9 flanking region of ZFX-ZFY genes.
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