Development of Ultra-rapid Multiplex Real-time PCR for the Detection of Genes from Avian Influenza Virus subtype H5N1
2007
Kim, E.H. (Kyonggi University, Suwon, Republic of Korea) | Lee, D.W. (Kyonggi University, Suwon, Republic of Korea) | Han, S.H. (Kyonggi University, Suwon, Republic of Korea) | Lim, Y.K. (Cheju National University, Jeju, Republic of Korea) | Yoon, B.S. (Kyonggi University, Suwon, Republic of Korea), E-mail: bsyoon@kyonggi.ac.kr
Cause of high lethality and dissemination to human being, new development of rapid method for the detection of highly pathogenic Avian Influenza Virus (AIV) is still necessary. For the detection of AIV subtype H5N1, typical pathogenic AIV, new method to confirm sub-typing of this virus is also needed. For the purpose of ultra-rapid detection and sub-typing of hemagglutinin and neuraminidase of AIV, this study was planned. As the results we could demonstrate an ultra-rapid multiplex real-time PCR (URMRT-PCR) for the detection of AIV.
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