Fatty acid composition of the phylum charophyta from Sindh, Pakistan
2007
Valeem, E.E. (Government Degree Science and Commerce Coll., Karachi (Pakistan). Dept. of Botany) | Shameel, M. (Karachi Univ. (Pakistan). Dept. of Botany)
Five species of calcified, stonewort algae belonging to two genera, Chara and Nitella were collected from different brackish water habitats at Gadap, Hyderabad, Kinjhar Lake, Kotri and Petaro during October 1997 and May 1998. They were extracted in chloroform:methanol, saponified, subjected to column chromatography (CC, TLC), esterified and analyzed for fatty acid (FA) composition initially by gas-liquid-chromatography (GLC) and finally by gas chromatography-mass spectrometry (GC-MS). The investigated algae possessed small number of SCFAs, VLCFAs, PUFAs, monoynoic and substituted FAs, no CFA and DCFA, small quantity of C16:0, medium amount of C18:1 acids, C18 UFAS up to 5 DBs, C20 UFAS up to 6 DBs, C22 UFAS up to 4 DBs, medium RCCL and FA-diversity. They were characterized by the largest unsaturation of C20 UFAs, the small number of occurrence and the medium size of most of the variables of FA-diversity.
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